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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -RNA Polymerase II (MGC75453, B220, DNA-directed RNA Polymerase II Largest Subunit, hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA Polymerase II (DNA directed) Polypeptide A 220kD, RPB1, RPB220, RPBh1, RpIILS, RPO2, RPOL2)

Anti -RNA Polymerase II (MGC75453, B220, DNA-directed RNA Polymerase II Largest Subunit,
hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA Polymerase II (DNA directed) Polypeptide A
220kD, RPB1, RPB220, RPBh1, RpIILS, RPO2, RPOL2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Catalog #R2039-19
ApplicationsSuitable for use in Immunoblot (Western) and Immunohistochemistry (frozen tissue).
Recommended DilutionImmunohistochemistry: 1:100 (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min). Incubation Time 1 hr at RT.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F159
HostMouse
ConcentrationNot determined
FormSupplied as a liquid.
PurityCulture supernatant
ImmunogenRNA polymerase II, 794DDYGPESRGFVENSYLA822
SpecificityExcellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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