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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -RNA Polymerase II (Rpb1 subunit, CTD, 220kD, Pol2)

Anti -RNA Polymerase II (Rpb1 subunit, CTD, 220kD, Pol2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse B IP
RNA polymerase II is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair (1,2). The largest subunit, Rpb1, contains a unique heptapeptide: Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7 , which is repeated up to 52 times in the carboxy-terminal domain (CTD) of Rpb1 (3). This CTD is phosphorylated by cyclin-dependent kinases (cdks), p44/42 MAPK, DNA-PKcs, and c-Abl (4). DNA damage caused by UV-light, hydrogen peroxide, or cisplatin results in ubiquitination and proteasomal degradation of Rpb1 (1,3). The kinase inhibitor 5,6-dichloro-1-b-Dribofuranosylbenzimidazole prevents ubiquitination of Rpb1, suggesting that CTD phosphorylation is required for proteolysis (5).
Catalog #R2039-22N
ApplicationsSuitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blotting 1:1000
Immunoprecipitation 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8A33
HostMouse
SourceHuman
FormSupplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PurityAs reported
ImmunogenSynthetic phosphopeptide (KLH-coupled) containing 10 heptapeptide repeats [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] in which Ser5 is phosphorylated.
SpecificityDetects endogenous levels of total Rpb1 protein (both phosphorylated and unphosphorylated forms).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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