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You are here:Home » Antibodies » Antibodies-Enzymes, Polymerase » Anti -RNA Polymerase II, CTD Repeat (YSPTSPS) (RNA Pol II CTD, RNA Pol II-CTD, DNA-directed RNA Polymerase II Largest Subunit, RPB1, Pol2)

Anti -RNA Polymerase II, CTD Repeat (YSPTSPS) (RNA Pol II CTD, RNA Pol II-CTD,
DNA-directed RNA Polymerase II Largest Subunit, RPB1, Pol2)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IF
RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Catalog #R2039-22R
ApplicationsSuitable for use in Immunofluorescence, ELISA Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9C82
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by Protein A affinity chromatography.
Immunogen10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human).
SpecificityThis antibody recognizes the C-terminal repeat of the largest subunit of RNA polymerase II, it recognizes both unphosphorylated and phosphorylated forms. Species Crossreactivity: Crossreacts with Human, S. cerevisiae. Expected to crossreact with Drosophilia melanogaster, Hamster, Mouse, S. pombe, Arabidopsis thaliana due to sequence homology - all of these species contain at least one or more 100% identical repeats.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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