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You are here:Home » Molecular Biology » MB-Cloning-Enzymes » RNA Polymerase, T7

RNA Polymerase, T7


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Catalog #R2049-05
Storage Buffer20mM Potassium Phosphate (pH 7.9), 1mM DTT, 0.1mM EDTA, 100mM NaCl and 50% glycerol.
Reaction Buffer (5X)200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl, 50mM NaCl, 10mM spermidine.
Unit DefinitionOne unit is the amount of enzyme that incorporates I nmole of [3H] GMP into acid-insoluble fraction in 1 hour at 37°C and pH 8.
PurityNuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis:
1. After incubation of 1ug of DNA-Hind III fragments with 500 units of enzyme for 24 hours at 37°C
2. After incubation of 1ug of pBR322 DNA with 500 units of enzyme for 24 hours at 37°C
3. After incubation of 1ug of 16S and 23S rRNA with 500 units of enzyme for 24 hours at 37°C This enzyme was more than 95% homogeneous as judged from SDS-polyacrylamide gel electrophoresis.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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