USBio Logo

R2997 Human Anti-Ro/SSA

Specifications
References
Clone Type
Polyclonal
Host
Human
Source
Human
Isotype
IgG
Grade
Purified
Applications
E
Crossreactivity
Bo Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

The Ro/SSA antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the Ro/SSA antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-Ro/SSA autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the Ro/SSA antigen may be used as a diagnostic aid. Elevated levels of Anti-Ro/SSA have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-Ro/SSA and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with Ro/SSA precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-Ro/SSA have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.

Applications
Suitable for use in ELISA and Immunodiffusion. Other applications not tested.
Recommended Dilutions
ELISA: 1:100 Optimal dilutions to be determined by the researcher.
Titer
Immunodiffusion: 1:4
Ro/SSA Autoantibody Activity (by ELISA)
6447EU/ml (lot-specific; value may differ from lot to lot).
Other Autoantibody Activity in the Serum (by ELISA)
SSB: 3.34EU/ml SCL: 3.17EU/ml Sm: 2.55EU/ml Sm/RNP: 2.74EU/ml Jo-1: 2.97EU/ml
Interpretation of ELISA Results
Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
This product is Ro/SSA autoantibody derived from human serum.
Form
Supplied as a lyophilized powder from PBS (0.01M phosphate/0.13M sodium chloride), pH 7.2, 0.02% sodium azide. Reconstitute with 2ml ddH2O.
Purity
Purified by fractionation of delipidated and defibrinated donor human serum.
Specificity
Recognizes human Ro/SS-A Antigen. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified La/SS-B, Sm, Scl-70, Sm/RNP, Jo-1, Mitochondrial or Ribosomal P antigens in an ELISA system designed to detect IgG antibody. Species Crossreactivity: bovine
References
1. Mamula MJ, Fox OF, Yamagata H, and Harley JB. "The (Ro) SS-A Autoantigen as an Immunogen Some Anti-(Ro) SSA Antibody Binds IgG." J of Exp Med Vol 86: pg 1889-1901. 2. Rader MD, O'Brien C, Liu Y, Harley JB, and Reichlin M. "Heterogeneity of the (Ro) SSA Antigen, Different Molecular Forms in Lymphocytes and Red Blood Cells." J. Clin. Invest Vol 83: 1989, pg 1293-1298. 3. Harley JB, Kaine JL, Fox OF, Reichlin M, Gruber B. "Ro SSA Antibody and Antigen in Cogenital Complete Heart Block ((Ro) SSA and Heart Block)." University of Oklahoma, Health Science Center, Arthritis and Immunology Program, Oklahoma Medical Research Foundation, pg 1-16. 4. Harley JB, Alexander EL, Bias WB, Fox OF, et al. "Anti-(Ro) SSA and Anti-(La) SSB in Sjogren's Syndrome." Dept of Med, University of Oklahoma, Health Science Center, Arthritis and Immunology Program, Oklahoma Medical Research Foundation, pg 1-35.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved