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Specifications

Clone Host Grade Applications Polyclonal Human Purified E

The Ro/SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the Ro/SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-Ro/SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the Ro/SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-Ro/SS-A have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-Ro/SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with Ro/SS-A precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-Ro/SS-A have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.
Catalog #	R2997
Applications	Suitable for use in ELISA and Immunodiffusion. Other applications not tested.
Recommended Dilution	ELISA: >1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute in 2ml sterile ddH2O.
Purity	Purified by delipidation and defibrination followed by salt fractionation.
Immunogen	Ro/SS-A antigen. Autoantibody derived from human serum.
Specificity	Recognizes human Ro/SS-A Antigen, demonstrates a strong Anti-Ro/SS-A precipitin band. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Exhibits strong positive in an Anti-Ro/SS-A ELISA system designed to detect all IgG subclasses. Does not crossreact at a dilution of 1
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.