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Specifications

Clone Host Grade Applications Polyclonal Human Purified E

The Ro/SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the Ro/SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-Ro/SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the Ro/SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-Ro/SS-A have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-Ro/SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with Ro/SS-A precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-Ro/SS-A have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.
Catalog #	R2997
Applications	Suitable for use in ELISA and Immunodiffusion. Other applications not tested.
Recommended Dilution	ELISA: >1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute in 2ml sterile ddH2O.
Purity	Purified by delipidation and defibrination followed by salt fractionation.
Immunogen	Ro/SS-A antigen. Autoantibody derived from human serum.
Specificity	Recognizes human Ro/SS-A Antigen, demonstrates a strong Anti-Ro/SS-A precipitin band. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Exhibits strong positive in an Anti-Ro/SS-A ELISA system designed to detect all IgG subclasses. Does not crossreact at a dilution of 1
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.