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You are here:Home » Antibodies » Antibodies-Autoimmune » Anti -Ro/SS-A Antigen

Anti -Ro/SS-A Antigen


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Clone Host Grade Applications
Polyclonal Human Purified E
The Ro/SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the Ro/SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-Ro/SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the Ro/SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-Ro/SS-A have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-Ro/SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with Ro/SS-A precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-Ro/SS-A have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.
Catalog #R2997
ApplicationsSuitable for use in ELISA and Immunodiffusion. Other applications not tested.
Recommended DilutionELISA: 1:100
Optimal dilutions to be determined by the researcher.
TiterImmunodiffusion: 1:16
Other Autoantibody Activity in the SerumThe serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSB, SCL, Sm, Sm/RNP, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses.
SSA 9250EU/ml
SSB: 2EU/ml
SCL: 2EU/ml
Sm: 2EU/ml
Sm/RNP: 4EU/ml
Jo-1: 4EU/ml
Interpretation of ResultsNegative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.13M sodium chloride, 0.02% sodium azide. Reconstitute with 2ml ddH2O.
PurityPurified by fractionation of delipidated and defibrinated donor human serum.
ImmunogenRo/SS-A antigen. Autoantibody derived from human serum.
SpecificityRecognizes human Ro/SS-A Antigen. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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