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You are here:Home » Antibodies » Abs to Infectious Disease Rotavirus » Anti -Rotavirus, ICP, Late Structural Virion Antigens (FITC)

Anti -Rotavirus, ICP, Late Structural Virion Antigens (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified IF
Catalog #R3000-01F
ApplicationsSuitable for use in Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.
Titer~1:250 by Indirect Immunofluorescence
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~4-5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified IgG fraction to a purity of > 95%.
ImmunogenBovine (Nebraska Calf Diarrhea Virus)
SpecificityRecognizes ICPs and late structural (virion) antigens. Reacts >90% with human rotaviruses. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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