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You are here:Home » Antibodies » Abs to Infectious Disease Rotavirus » Anti -Rotavirus, ICP, Late Structural Virion Antigens (HRP)

Anti -Rotavirus, ICP, Late Structural Virion Antigens (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E B IH
Catalog #R3000-06
ApplicationsImmunohistochemistry, Western Blot and ELISA.
Recommended DilutionWestern Blot:1:20-1:200
Immunohistochemistry:1:20-1:200
ELISA: 1:200-1:1000. Titer: ~1:250 by indirect immunofluorescence
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypePolyclonal
HostGoat
Concentration1–2 mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% Thimerosal. Conjugated to a highly purified preparation of horseradish peroxidase (RZ>3).
PurityPurified IgG fraction. Free enzyme is absent. Estimated molar HRP: IgG substitution is 2-3.
ImmunogenBovine (Nebraska Calf Diarrhea Virus)
SpecificityNegative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence. 90% with human rotaviruses (reported). ICPs + late structural (virion) antigens
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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