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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E IF

Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present.
Catalog #	R3000-08B
	Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
Applications	Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	9L628
Host	Mouse
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.1% sodium azide.
Purity	Purified by DEAE chromatography.
Immunogen	Electron microscopy characterized wild strain, isolated from calf feces.
Specificity	Recognizes rotavirus on bovine and human feces. Recognizes the 41kD capsid major protein, group specific antigen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.