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Specifications

Clone Host Grade Applications Polyclonal Goat Highly Purified IF

Rotavirus is a virus that infects the bowels and is the most common cause of gastroenteritis in infants. 7 major groups have currently been identified, 3 of which infect humans (groups A, B and C). Its genome consists of 11 segments of double stranded RNA held in the inner core of the three layered, icosahedral virion particle. The genome codes for 6 virus proteins (VP1,2,3,4,6,7) and 6 non-structural proteins (NSP1-6). Outer capsid proteins are VP4 and VP7, inner capsid -VP6 and core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection.
Catalog #	R3000-13
Applications	Suitable for use in Immunofluorescence. Other applications not tested.
Recommended Dilutions	Direct Immunofluorescence: 1:20-1:100
	Indirect Immunofluorescence:1:500; Acetone fixation of the antigen is recommended.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with FITC.
Purity	Purified by chromatography.
Immunogen	Rotavirus (whole virus)
Specificity	Recognizes Rotavirus (whole virus): 100%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.