Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein
Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD
Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Prote
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| Recombinant full-length rat RSK1 (residues 1–end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags. Expressed in baculovirus in Sf21 insect cells | | | Catalog # | R9399-08A | | Specific Activity | ~4000-5000U/mg, where one unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide per minute at 30°C with a final ATP concentration of 100uM. | | Quality Control Testing | Kinase Assay: 0.3–3.8ng phosphorylates 30uM RSKtide. Assay background was subtracted from the actual counts to yield the results shown below. | | MS Tryptic Fingerprint: Confirmed product identity as rat RSK1 with the translated sequence. SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of RSK1 active. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Handling Recommendations | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C. | | Molecular Weight | 89.9kD | | Source | Recombinant, rat expressed in Sf21 insect cells | | Purity | ~95%. Purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies. | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Prote |
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