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You are here:Home » Molecular Biology » MB-Protein Kinases, Ribosomal » RSK1, active, Recombinant, Human (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Pr

RSK1, active, Recombinant, Human (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6
Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a,
MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Pr

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Specifications

N-terminal His6 tagged recombinant full-length human RSK1 expressed in Sf21 cells. Activated
with PDK1 and MAP Kinase 2/Erk2 and repurified using glutathione-Sepharose, heparin-Sepharose and Ni2+/NTA agarose.
Catalog #R9399-10
Specific Activity~2900U/mg,
Unit DefinitionOne unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM M2355-09 MAPKAP Kinase 2 Substrate Peptide (KKLNRTLSVA) per minute at 30°C with a final ATP concentration of 100uM.
Identity ConfirmationMS Tryptic Fingerprint: Confirmed identity as RSK1 with 38% amino acid coverage of the translated sequence.
ApplicationsKinase Assay: Kinase Assay: 0.3-2.5ng phosphorylated 30uM MAPKAP Kinase 2 Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight84kD
SourceSf21 cells
Purity~94% by SDS-PAGE and Coomassie blue staining.
Concentration~0.1mg/ml
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride pH 7.5, 0.03% Brij 35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine and 0.1% 2-mercaptoethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesRibosomal Protein S6 Kinase 90kDa Polypeptide 1, EC=2.7.11.1, HU-1


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