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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)

Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IC
Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581). There is one alternate start site at Met117. Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk.
Catalog #R9951-50
ApplicationsSuitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived, recombinant mouse Ryk, extracellular domain (aa35-211, Accession #Q01887).
SpecificityRecognizes mouse Ryk. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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