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You are here:Home » Antibodies » Antibodies-Tyrosine Kinases » Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)

Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IC
Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581). There is one alternate start site at Met117. Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk.
Catalog #R9951-50
ApplicationsSuitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived, recombinant mouse Ryk, extracellular domain (aa35-211, Accession #Q01887).
SpecificityRecognizes mouse Ryk. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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