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Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)
Anti -Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IC |
|
| Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581). There is one alternate start site at Met117. Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk. | | | Catalog # | R9951-50 | | Applications | Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested. | | Recommended Dilutions | Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well. | | Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA. | | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | NS0-derived, recombinant mouse Ryk, extracellular domain (aa35-211, Accession #Q01887). | | Specificity | Recognizes mouse Ryk. Species Crossreactivity: human | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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