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S1 Nuclease, A. orzyae, ss-DNA degradation, Solution
S1 Nuclease, A. orzyae, ss-DNA degradation, Solution
Pricing
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| S1 Nuclease degrades single-stranded DNA and RNA yielding 5’-phosphoryl-terminated products. | | | Catalog # | S0050-01 | | Applications | • Removal of protruding single-stranded termini in double-stranded DNA. | | • Selective cleavage of single-stranded DNA | | • Mapping RNA transcripts | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~34kD | | Source | Aspergillus orzyae | | | Specificity | Ss-DNA degradation. Activity on native dsDNA undetectable under the assay conditions. | | | Concentration | 100,000 units/ml | | | Form | Supplied as a liquid in 30mM sodium acetate, pH 4.6, 50mM sodium chloride, 1mM zinc chloride, 50% glycerol. | | | Quality Control | No double stranded DNA digestion was detected after incubation of 10units of S0050-01 with 1ug pUC 19 for 1hour at 37°C. | | | Unit Definition | | | One unit hydrolyses 1ug of nucleic acid per minute at 37ºC, pH 4.6. |
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