Anti -S6K1 Aly/REF-like Target alpha, beta (SKAR, 46kD DNA Polymerase delta Interaction
Protein, p46, PDIP46, KIAA1649, Polymerase delta-interacting Protein 3, POLDIP3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B IF |
|
| p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, a and b, the latter having a 29aa truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3). | | | Catalog # | S0050-85 | | Applications | Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. | | Recommended Dilution | Immunofluorescence (IF-IC): 1:3200 | | Western Blot: 1:1000 Icubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 11C1067 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to the amino terminus of human SKAR. | | Specificity | Recognizes endogenous levels of total SKAR protein, A/B isoforms. Species Crossreactivity: monkey, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Polymerase (DNA-directed), delta Interacting Protein 3 |
External Links
|
