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You are here:Home » Antibodies » Abs to Disease Markers » Anti -S100 Calcium Binding Protein A9 (S100A9, 60B8AG, Calgranulin B, CAGB, Calprotectin L1H Subunit, CFAG, CGLB, Cystic Fibrosis Antigen, L1AG, Leukocyte L1 Complex Heavy Chain, LIAG, MAC387, Migration Inhibitory Factor-related Protein 14, MIF Related Protein

Anti -S100 Calcium Binding Protein A9 (S100A9, 60B8AG, Calgranulin B, CAGB, Calprotectin
L1H Subunit, CFAG, CGLB, Cystic Fibrosis Antigen, L1AG, Leukocyte L1 Complex Heavy Chain,
LIAG, MAC387, Migration Inhibitory Factor-related Protein 14, MIF Related Protein

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.
Catalog #S0051-17J4
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionPeptide ELISA: 1:8,000.
Western Blot: 0.01-0.03ug/ml. A Band is detected at ~16kD in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kDa according to NP_002956.1). Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See S0051-17J4-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of aa-DTNADKQLSFEEF.
SpecificityRecognizes human S100A9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesS100A9; S100 calcium binding protein A9; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14


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