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You are here:Home » Antibodies » Abs to Calcium Binding Proteins » Anti -S100 Calcium Binding Protein A4 (S100A4)

Anti -S100 Calcium Binding Protein A4 (S100A4)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
Catalog #S0051-84
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant mouse S100A4.
SpecificityRecognizes mouse S100A4. Less than 1% cross-reactivity with S100A8, A9 and A10. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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