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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -S100 Calcium Binding Protein (S100-beta, Calcium Binding Protein B1)

Anti -S100 Calcium Binding Protein (S100-beta, Calcium Binding Protein B1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Protein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumors whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao).
Catalog #S0052-06C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry (formalin-fixed and paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G435
HostMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenBovine kidney S100 beta.
SpecificityRecognizes S100 beta. Species Crossreactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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