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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Used for the detection of salmonid parasites (bacteria or viruses), indirectly by detecting parasite specific immunoglobulins from Salmo, Oncorhynchus and Salvelinus salmon. Also used for the detection of unknown parasites by monitoring the level of serum immunoglobulins in salmonid stock.
Catalog #	S0059-07B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:500-1:1000
	Western Blot: 1:400-1:800
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	2J3
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Purity	Purified by Protein G affinity chromatography
Immunogen	Rainbow Trout and Atlantic Salmon heavy and light chain immunoglobulins.
Specificity	Recognizes Salmo, Oncorhynchus and Salvelinus serum immunoglobulins. Does not recognize China Perch or Zebra Fish.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.