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You are here:Home » Antibodies » Abs to Fish » Anti -Salmon (Salmonid, a-SIg) (HRP)

Anti -Salmon (Salmonid, a-SIg) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Used for the detection of salmonid parasites (bacteria or viruses), indirectly by detecting parasite specific immunoglobulins from Salmo, Oncorhynchus and Salvelinus salmon. Also used for the detection of unknown parasites by monitoring the level of serum immunoglobulins in salmonid stock and responses to specific parasites, or to assess the efficacy of salmonid vaccines.
Catalog #S0059-07B
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:500-1:1000
Western Blot: 1:400-1:800
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2J3 (5-F12)
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
PurityPurified by Protein G affinity chromatography
ImmunogenRainbow Trout and Atlantic Salmon heavy and light chain immunoglobulins.
SpecificityRecognizes Salmo, Oncorhynchus and Salvelinus serum immunoglobulins. Reacts with Atlantic Salmon, Brown Trout,
Chinook Salmon, Chum Salmon, Coho Salmon, Sockeye Salmon, Rainbow Trout, Arctic Char, Brook Trout. Does not recognize China Perch or Zebra Fish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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