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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Catalog #	S0060-10C
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:10
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	9L639
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Supernatant
Immunogen	Flagellar preparation from S. hadar.
Specificity	Recognizes S. hadar-100%; S. typhimurium (phase 1)- 48%; S. typhimurium (phase 2)-137%; S. enteritidis-47%; S. virchow-40%; S. bredeney-55%; S. weltevreden-80%; S. infantis-91%; S. agona-31%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.