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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IF

The O antigen is a polysaccharide that extends from the core oligosaccharide, which itself is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs. The O antigen is the outermost component of the LPS and functions as a receptor for bacteriophages. It is also important in the immune response of the host. Considerable diversity is seen within Salmonella O antigens.
Catalog #	S0060-18B
	Salmonella enterica, serovar paratyphi A belongs to serogroup A of Salmonella and causes paratyphoid fever, which is similar to typhoid fever but more benign.
Applications	Suitable for use in ELISA, Immunoblot, and Immunofluorescence. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10F421
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Heat inactivated cells of Salmonella paratyphi A.
Specificity	Recognizes the O antigen of Salmonella enterica, serovar paratyphi A (also known as S. paratyphi), and it is specific for the 0-2 antigenic determinant of Salmonella serogroup A and does not cross-react with the LPS of serogroups B, D or E of Salmonella spp.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.