| The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs. |
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| Catalog # | S0060-26H |
| Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses. |
| Applications | Suitable for use in ELISA, Dot Blot and Immunofluorescence. Other applications not tested. |
| Recommended Dilutions | Optimal dilutions to be determined by the researcher. |
| Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 10F416 |
| Host | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Heat inactivated Salmonella. |
| Specificity | Recognizes the core antigen of Salmonella enterica, serogroups A (S. paratyphi A), B (S. typhimurium), C1 (S. choleraesuis), C2 (S. newport), D (S. enteriditis), E1 (E. anatum) and E2 (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
