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You are here:Home » Antibodies » Abs to Infectious Disease Salmonella » Anti -Salmonella typhimurium LPS

Anti -Salmonella typhimurium LPS

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IF
Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the intestinal lumen. Its toxicity is due to an outer membrane consisting largely of lipopolysaccharides (LPS) which protect the bacteria from the environment. The LPS is made up of an O-antigen, a polysaccharide core, and lipid A, which connects it to the outer membrane. Lipid A is made up of two phosphorylated glucosamines which are attached to fatty acids. These phosphate groups determine bacterial toxicity. Animals carry an enzyme that specifically removes these phosphate groups in an attempt to protect themselves from these pathogens. The O-antigen, being on the outermost part of the LPS complex is responsible for the host immune response. S. typhimurium has the ability to undergo acetylation of this O-antigen, which changes its conformation, and makes it difficult for antibodies to recognize.
Catalog #S0060-32D
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F423
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified LPS from S. typhimurium.
SpecificityRecognizes an unidentified LPS determinant of Salmonella typhimurium.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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