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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative and flagellated (motile).
Catalog #	S0060-40A
	Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:10
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	9L644
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Supernatant
Immunogen	Flagellar preparation of S. enteritidis.
Specificity	Recognizes S. enteritidis-100%; S. enteritidis PT4 strains-33-89%; S. agona-78%; S. typhimurium-5%; S. infantis-4%; S. hadar-1%; S. virchow-3%; Enterobacter cloacae-3%; E. coli-1%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.