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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Catalog #	S0060-40C
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10F419
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Heat inactivated cells from Salmonella (LPS groups A, B & D).
Specificity	Recognizes a common antigenic determinant that is found in the LPS of Salmonella enteriditis (Group D), Salmonella typhimurium (Group B) and Salmonella paratyphi (Group A). Thought to recognize the '0-12' antigen [2-D-mannose (1-4) I-ramnose (1-3)-D galactose] of Salmonella LPS. A similar sequence is found in Group E, which may account for the weak cross reactivity observed with bacteria of this group.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.