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You are here:Home » Antibodies » Abs to Infectious Disease Salmonella » Anti -Salmonella enteritidis LPS

Anti -Salmonella enteritidis LPS

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Catalog #S0060-40C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F419
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHeat inactivated cells from Salmonella (LPS groups A, B & D).
SpecificityRecognizes a common antigenic determinant that is found in the LPS of Salmonella enteriditis (Group D), Salmonella typhimurium (Group B) and Salmonella paratyphi (Group A). Thought to recognize the '0-12' antigen [2-D-mannose (1-4) I-ramnose (1-3)-D galactose] of Salmonella LPS. A similar sequence is found in Group E, which may account for the weak cross reactivity observed with bacteria of this group.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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