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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

Anti -SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase,
Jun-terminal Kinase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP FC IF
The stress-activated protein kinase/Junterminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1-4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.
Catalog #S0096-04C
ApplicationSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000. Incubate membrane with diluted S0096-04C in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:250
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.
Source Ascites
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3A33
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK (KLH coupled).
SpecificityDetects endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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