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S0096-25 SAPK2, p38 MAPK Inhibitor (SB 202190) (Stress-activated Protein Kinase 2) CAS: 152121-30-7

Specifications
References
CAS Number
152121-30-7
Grade
Highly Purified
Swiss Prot
Q15759
Accession Number
NM_002751.5
Molecular Formula
C20H14FN3O
Molecular Weight
331.3
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

SB 202190 is a cell permeable pyridinyl imidazole, which acts as a strong inhibitor of SAPK2a/p38 and SAPK2b/p38beta MAP kinases (IC50=280nM and 350nM, respectively). SB 202190 has no effect on the activity of the Erk or SAPK/JNK MAP kinases and is reported to block LPS induced TNF-a and IL-1beta production in mice. The compound is a slightly better inhibitor than the related compound SB 203580.

Solubility
Soluble in DMSO (50mg/ml).
Applications
Specific inhibitor of p38/SAPK2a MAPK and P38b/SAPK2a MAPK activity. Other applications not tested.
Protein Kinase Inhibition Assay
When pre-incubated for five minutes with 0.2ug of SAPK2a/p38 MAPK or SAPK2b/p38beta MAPK, 1-10uM of SB 202190 will inhibit the activity of these kinases 80-95%. The final concentration of SB 202190 needed may depend on the amount of enzyme used. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Protect from light.
Purity
~98% (HPLC)
Form
Supplied as a pale yellow solid.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Jiang, Y., et al., J. Biol. Chem. 271: 17,920 (1996). 2. Kramer, R.M., et al., J. Biol. Chem. 271: 27,723 (1996). 3. Li, Z., et al., Biochem. Biophys. Res Commun. 228: 334 (1996). 4. Lee, J.C., et al., Nature 372: 739 (1994).
USBio References
No references available
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