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You are here:Home » Molecular Biology » MB-Protein Kinases » SAPK2a, p38 alpha, active (Stress-activated Protein Kinase 2a) Recombinant

SAPK2a, p38 alpha, active (Stress-activated Protein Kinase 2a) Recombinant

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Specifications

GST fusion protein containing full length human SAPK2a/p38a (64kDa).
Catalog #S0096-28
Specificity Essentially identical in mammals (p38 MAPK) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.
Specific Activity 50units/mg using MBP as a substrate. One unit of SAPK2a/p38a = 1 nmol phosphate incorporated into MBP per minute.
SAPK2a Kinase Assay SAPK2a/p38a phosphorylated MBP.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman recombinant expressed in E. coli
Purity98% by SDS and Coomassie Blue staining
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.
Intended for research use only, not for use in human, therapeutic or diagnostic applications.


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