Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Protein Kinases » SAPK2b, p38 beta, active (Stress-activated Protein Kinase 2b) Recombinant

SAPK2b, p38 beta, active (Stress-activated Protein Kinase 2b) Recombinant


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Essentially identical proteins in mammals (p38 MAP Kinases) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.
Catalog #S0096-29
Recombinant, GST fusion-protein corresponding to full length human SAPK2b, expressed in E. coli. MW ~65kD
Specific Activity 55u/ug using MBP as a substrate (1 unit of SAPK2b/p38beta = 1unit of MAPKAP Kinase-2 generated per minute = 1nmol phosphate incorporated into myelin basic protein (MBP) per minute.)
Applications Coupled Kinase Assay Enzyme was tested in vitro in a coupled kinase phosphorylation assay using 20-100ng of active SAPK2b to activate GST-MAPKAP Kinase-2, unactivated as shown by phosphorylation of MAPKAP Kinase-2 Substrate Peptide.
Storage and Stability Stable for 6 months at -20°C from date of shipment.
Molecular Weight~65kD
SourceHuman recombinant
PurityPurified to >80% by affinity chromatography on glutathione-Sepharose™ beads.
Form5ug recombinant enzyme in 25ul of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

External Links