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You are here:Home » Antibodies » Abs to Splicing Factors » Anti -SAP145 (Spliceosome-associated Protein 145, SAP-145, Splicing Factor 3B Subunit 2, Pre-mRNA-splicing Factor SF3b 145kD Subunit, SF3b145, SF3b150) (Azide Free)

Anti -SAP145 (Spliceosome-associated Protein 145, SAP-145, Splicing Factor 3B Subunit 2,
Pre-mRNA-splicing Factor SF3b 145kD Subunit, SF3b145, SF3b150) (Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Catalog #S0096-50A
ApplicationsSuitable for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1.5ug/ml
Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B2481
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to a portion of amino acids within aa592-646 of human SF3B2.
SpecificityRecognizes human SAP145.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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