Anti -SAPK4, p38 delta (Stress-activated Protein Kinase 4, Mitogen-activated Protein
Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38
delta, MAP Kinase p38 delta, p38delta)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
|
E B |
|
| Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser-Pro or Thr-Pro peptide motifs. | | | Catalog # | S0096-54 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.05ug/ml | | Western Blot: 1ug/ml (Alkphos detection), 0.1ug/ml (HRP/ECL detection). | | Optimal dilutions to be determined by the researcher. | | Positive Control (included) | A0001-50B A431 cell lysate, untreated | | Add 75ul 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. | | Western Blot: 5ul/lane (mini gel) for HRP/ECL detection; 20ul/lane (mini gel) for HRP/DAB detection. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6D753 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, PEG, sucrose, 0.1% sodium azide. | | Purity | Purified by thiophilic adsorption and size-exclusion chromatography. | | Immunogen | Synthetic peptide corresponding to a portion of N-terminal SAPK4 (KLH). | | Specificity | Recognizes N-terminal human, mouse and rat p38 . Species Crossreactivity: Human, mouse, rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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