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You are here:Home » Antibodies » Abs to Infectious Disease SARS » Anti -SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)

Anti -SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
Catalog #S0098-13
ApplicationsSuitable for the detection of SARS Nucleocapsid protein in western blot analysis and ELISA. The antibody was tested on a cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46kD.
Recommended DilutionWestern blot: 1:100-1:2000
ELISA: 1:100-1:2000
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified
ImmunogenSynthetic peptides corresponding to amino acids 354-370 of putative SARS nucleocapsid (Genbank accession no. NP_828858).
SpecificityRecognizes SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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