You are here:Home
»
Antibodies
»
Abs to Infectious Disease SARS
»
Anti -SARS Nucleoprotein (Severe Acute Respiratory Syndrome)
Anti -SARS Nucleoprotein (Severe Acute Respiratory Syndrome)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IF |
|
| The Coronaviridae virus family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The N protein (nucleoprotein) is one of the major structural proteins in a viral particle, playing a critical role in the transcription regulation of the genomic RNA and other viral proteins. It might also be involved in the virulence and virus-specific post-translational modifications. The N protein is composed of 422 amino acids (a.a.) with an estimated molecular weight of 46.03 kD. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The N protein contains multiple candidate phosphorylation sites, suggesting that it may be related to important functions, such as | | | RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus. | | | Catalog # | S0098-19 | | Applications | Suitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested. | | Recommended Dilution | Immunofluorescence: 1-5ug/ml | | Western Blot: 1-5ug/ml | | ELISA: 0.1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 6A181 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide | | Purity | Purified by Protein A chromatography. | | Immunogen | Recombinant SARS (severe acute respiratory syndrome) nucleoprotein. | | Specificity | Recogizes the human SARS-CoV nucleoprotein. On Western blots, it identifies the target band at ~50kD. Does not crossreact with the nucleoprotein of 229E-CoV or DC43-CoV. Reactivity has also been confirmed with SARS infected MDCK cells by immunofluorscence. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
