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You are here:Home » Antibodies » Abs to Infectious Disease SARS » Anti -SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)

Anti -SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated
Coronavirus)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Catalog #S0098-35
ApplicationsSuitable for detection of SARS Spike protein in western blot analysis. The antibody was tested on a cell line transfected with full-length cDNA coding for the SARS Spike protein with a predicted molecular weight of 139kD. Western blot analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.
Recommended DilutionWestern blot: 1-3ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4H250
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified
ImmunogenSynthetic peptide from the putative SARS Spike glycoprotein (Genbank accession no. NP_828851) corresponding to amino acids 19-35.
SpecificityRecognizes SARS, Spike Protein (Severe Acute Respiratory Syndrome)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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