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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -Scavenger Receptor (SREC-I expressed by Endothelial Cells, Scavenger Receptor class F, member 1, SCARF1)

Anti -Scavenger Receptor (SREC-I expressed by Endothelial Cells, Scavenger Receptor class
F, member 1, SCARF1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B FC
Scavenger Receptor expressed by Endothelial Cells (SREC-I), also known as Scavenger Receptor class F, member 1 (SCARF1) is expressed on endothelial cells, macrophages and phagocytic cells. It contains 6 EGF-like repeats in its extracellular domain. Besides binding scavenger receptor ligands such as fucoidin and acetylated LDL, SREC-I is an endocytic receptor for calreticulin and gp96. It also trans-interacts with SREC-II through the extracellular EGF repeats.
Catalog #S0098-98
ApplicationsSuitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human SREC-I. The detection limit for rhSREC-I is approximately 1ng/well and 100ng/ml under non-reducing and reducing conditons, respectively.
Flow Cytometry 0.25ug/ml. Use 10ul of the diluted antibody to stain 1x10e5 to 2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. A fluorochrome-labeled secondary antibody such a goat anti-mouse IgG may be used for detection.
Western Blot 1-2ug/ml with the appropriate secondary reagents to detect human SREC-1. Using a colorimetric detection system, the detection limit for rhSREC-1 is approximately 1ng/lane and 100ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No8B43
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a powder lyophilized from a solution containing PBS with 5% trehalose. Reconstitute to 0.5mg/ml with 200ul of sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant protein comprising aa20-421 of human SREC-I (UniProt Accession #Q14162)
SpecificityRecognizes human SREC-I
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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