Schneiders Insect medium was developed and later modified by Dr. Schneider to support the growth and development of excised imaginal disks from the fruitfly, Drosophila melanogaster. The medium was later used to establish cell lines from Drosophila embryos. When supplemented with 5-20% heat-inactivated fetal bovine serum, Schneiders medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
Appearance
Off white to tan, homogenous, free flowing powder
Solubility
Yellow, clear, slight precipitate may form.
Directions per Liter
Dissolve 24.7g in 800-900ml of ddH2O stirring gently until completely solubilized. (water temperature should be 15-20°C). The powder will not go into solution completely. DO NOT heat. Add 0.4g/L sodium bicarbonate with sitrring. With stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH; liquid should clear. A bit of cloudiness may be observed due to excess calcium salt. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Inorganic Salts
| Calcium Chloride 0.6 |
| Magnesium Sulfate | 1.807221 |
| Potassium Chloride | 1.6 |
| Potassium Phosphate Monobasic | 0.45 |
| Sodium Bicarbonate | Absent |
| Sodium Chloride | 2.1 |
| Sodium Phosphate Dibasic | 0.7 |
Amino Acids
| ß-Alanine | 0.5 |
| L-Arginine | 0.6 |
| L-Aspartic Acid | 0.4 |
| L-Cysteine | 0.06 |
| L-Cystine•2HCl | 0.026732 |
| L-Glutamic Acid | 0.8 |
| L-Glutamine | 1.8 |
| Glycine | 0.25 |
| L-Histidine | 0.4 |
| L-Isoleucine | 0.15 |
| L-Leucine | 0.15 |
| L-Lysine | 1.65 |
| L-Methionine | 0.15 |
| L-Proline | 1.7 |
| L-Serine | 0.25 |
| L-Threonine | 0.35 |
| L-Tryptophan | 0.1 |
| L-Tyrosine•2Na•2H2O | 0.720199 |
| L-Valine | 0.3 |
Other
| Fumaric Acid | 0.06 |
| D(+)-Glucose | 2 |
| a-Ketoglutaric Acid | 0.35 |
| L(-)-Malic Acid | 0.6 |
| Succinic Acid | 0.06 |
| D(+)-Trehalose | 2 |
| Yeast Extract | 2 |
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