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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Sarcoplasmic Reticulum, Junctional

Anti -Sarcoplasmic Reticulum, Junctional

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IF
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR?s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRs), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95kD) transmits electrochemical signals to the SR through direct interaction with both DHPRs and RyRs. Another skeletal muscle specific 90kD protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.
Catalog #S0170
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:250; Immunofluorescence staining of junctional sarcoplasmic reticulum results in increased intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle
Western Blot: 1:1500; Detects an ~90kD protein representing junctional SR in rabbit skeletal muscle extracts.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i391
HostMouse
SourceRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityAscites.
ImmunogenRabbit skeletal muscle triads.
SpecificityDetects Junctional Sarcoplasmic Reticulum in skeletal muscle. It does not crossreact with cardiac muscle. Crossreacts strongly with Rabbit and Guinea pig, moderately with canine and Rat and weakly with Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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