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You are here:Home » Molecular Biology » Growth Factors-Cell Factors » SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)

SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand,
DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor,
SF)

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Specifications

Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Catalog #S0400-07P
Amino Acid SequenceThe sequence of the first five amino acids was determined to be Met-Glu-Gly-Ile-Cys
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <2ng/ml, corresponding to a Specific Activity of 5x10e5IU/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.4kD
SourceHuman recombinant SCF, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 165 amino acids.
PurityPurified by chromatography.
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 10mM acetic acid. Reconstitute with 20ul sterile ddH2O. For long term storage, add a carrier protein (0.1% HSA or BSA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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