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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -Scl-70 (Scleroderma, DNA Topoisomerase I)

Anti -Scl-70 (Scleroderma, DNA Topoisomerase I)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Topoisomerase I changes the topology of DNA via cleavage of single strand only. Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA.
Catalog #S0500-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4j81
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenFull length Scl-70. Epitiope: YFVPPKEDFK.
SpecificityRecognizes human Scl-70.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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