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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » SdaI (Sse8387I)

SdaI (Sse8387I)

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Specifications

5'-C C T G C A^G G-3'
3'-G G^A C G T C C-5'
Catalog #S0549
Concentration~10u/ul
Unit Definition One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA fragments in 1 hour at 37°C in 50ul of assay buffer.
SourceE. coli that carries the cloned sdaIR gene from Streptomyces diastaticus Ng 7-324
Supplied WithR1625: Restriction Enzyme Buffer A, 10X
R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X
Diluent BufferFor short term storage (~3-4 weeks)-10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.
Storage Buffer10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 5-fold overdigestion (5 units/ug lambda DNA x 1 hour) with SdaI (see Star Activity).
Ligation/Recutting AssayAfter 5-fold overdigestion (2.5 units/ug DNA x 2 hours) with SdaI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.05uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Blue/White Cloning AssayPUC19 DNA was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.1% of white colonies were detected.
Enzyme Properties
Star ActivityAn excess of enzyme
(7.5u/ug DNA x 1 hour) may result in star activity.
Methylation Effects
Dam/Dcm/CpG/EcoKl/EcoBlNever overlaps - no effect.
Stability During Prolonged IncubationA minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible EndsAlw21I, Mph1103I, PstI, SduI
Number of Recognition Sites in DNA
Lambda5
PhiX1740
M13mp18/191
pBR3220
pUC18/191
pUC570
pTZ19R/U1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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