| T T A A 5'-G G G C C^C-3' |
|
| 3'-C^C C G G G-5' T T A A |
|
| Catalog # | S0577 |
| Concentration | 10u/ul |
| Source: Streptococcus durans RFL3 |
| Supplied with | Catalog No: R1625-75: Restriction Enzyme Buffer for SduI, 10X |
| 10mM Tris-HCl (pH 7.2), 3mM MgCl2, 150mM NaCl and 0.1mg/ml BSA. Incubate at 37°C |
| Diluent Buffer | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. |
| Storage Buffer | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. |
| Overdigestion Assay | No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SduI (see Star Activity). |
|
| Ligation/Recutting Assay | After 50-fold overdigestion (3u/ug DNA x 17 hours) with SduI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.4uM. More than 95% of these can be recut. |
|
| Labeled Oligonucleotide (LO) Assay | No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. |
|
| Star Activity | A large excess of enzyme (10u/ug DNA x 16 hours), low salt concentration or high pH may result in star activity. |
|
| Stability during Prolonged Incubation | A minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. |
|
| Thermal Inactivation | Enzyme is inactivated by incubation at 65°C for 20min. |
|
| Compatible Ends | Alw21I, ApaI, Eco24I, Mph1103I, PstI, SacI, SdaI |
|
| Number of Recognition Sites in DNA | |
|
| Lambda | 38 |
| PhiX174 | 3 |
| M13mp18/19 | 5 |
| pBR322 | 10 |
| pUC18/19 | 5 |
| pUC57 | 6 |
| pTZ19R/U | 5 |
| pBluescriptIIKS(-/+) | 6 |
| pBluescriptIISK(-/+) | 6 |
| pACYC177 | 4 |
| pACYC184 | 8 |
|
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. |
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