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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » SduI

SduI

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Specifications

T T A A 5'-G G G C C^C-3'
3'-C^C C G G G-5' T T A A
Catalog #S0577
Concentration 10u/ul
Source: Streptococcus durans RFL3
Supplied withCatalog No: R1625-75: Restriction Enzyme Buffer for SduI, 10X
10mM Tris-HCl (pH 7.2), 3mM MgCl2, 150mM NaCl and 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with SduI (see Star Activity).
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with SduI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.4uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Star ActivityA large excess of enzyme (10u/ug DNA x 16 hours), low salt concentration or high pH may result in star activity.
Stability during Prolonged IncubationA minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Compatible EndsAlw21I, ApaI, Eco24I, Mph1103I, PstI, SacI, SdaI
Number of Recognition Sites in DNA
Lambda38
PhiX1743
M13mp18/195
pBR32210
pUC18/195
pUC576
pTZ19R/U5
pBluescriptIIKS(-/+)6
pBluescriptIISK(-/+)6
pACYC1774
pACYC1848
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


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