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You are here:Home » Antibodies » Abs to Proteins » Anti -Secretory Component

Anti -Secretory Component

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Secretory Component is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas.
Catalog #S0630
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin-fixed, paraffin-embedded): 1:100-1:200 for 20 minutes at RT. To stain formalin-fixed tissues, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, then cooling at RT for 20 minutes.
Optimal dilution determined by the researcher.
Positive ControlSmall intestine, stomach, colon carcinoma
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.556
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSemi-purified human secretory component (SC). Cellular Localization: Cytoplasmic.
SpecificityRecognizes a single band of 80kD human glycoprotein, identified as secretory component. Reacts with both free and polymeric Ig-bound secretory component. Shows no reaction in Western Blots of cell lines lacking secretory component. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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