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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Secretory Component

Anti -Secretory Component

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum E
Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.
Catalog #S0630-01H
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended DilutionsImmunoelectrophoresis: 120ul against 2ul serum or equivalent
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PuritySerum
ImmunogenPurified free human secretory component isolated from pooled milk.
SpecificityRecognizes human Secretory Component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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