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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E

Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free
form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific
mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain
and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.
Catalog #	S0630-01J
Applications	Suitable for use in ELISA, Immunoelectrophoresis, Single and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution	Immunoelectrophoresis: 120ul against 2ul serum or equivalent
	Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Monkey
Concentration	Not determined
Form	Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity	Serum
Immunogen	Purified free secretory component isolated from pooled rhesus monkey milk
Specificity	Recognizes bound secretory component (secretory IgA) and free secretory component present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.