| Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free |
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| form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific |
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| mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain |
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| and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. |
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| Catalog # | S0630-01N |
| Applications | Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. |
| Recommended Dilution | ELISA: 1:200-1:4000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Monkey |
| Concentration | Not determined |
| Form | Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Horseradish Peroxidase (HRP). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2. |
| Purity | Purified by DEAE chromatography. |
| Immunogen | Free monkey secretory component isolated from pooled milk. |
| Specificity | Recognizes monkey secretory component. Reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal |
| monkey milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alpha-2 region. Des not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion. May also react with free and bound secretory component in many old-world monkeys, including Macaca |
| Sylvanus, Cercopithecus, Cynomolgus, Baboon and chimpanzees. In double radial immunodiffusion a reaction has been observed with free human secretory component. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
