| Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free |
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| form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific |
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| mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain |
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| and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. |
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| Catalog # | S0630-01P |
| Applications | Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Rhodamine/IgG protein molar ratio (F/P) | ~1.5 |
| Excitation | 554nm |
| Emission | 573nm |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Rhodamine conjugates are light sensitive. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Monkey |
| Concentration | Not determined |
| Form | Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Rhodamine. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2. |
| Purity | Purified by DEAE chromatography. |
| Immunogen | Purified free monkey secretory component isolated from pooled milk. |
| Specificity | Recognizes monkey secretory component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory |
| component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
