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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Secretory Leukocyte Protease Inhibitor (SLPI)

Anti -Secretory Leukocyte Protease Inhibitor (SLPI)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH N
Human Secretory Leukocyte Protease Inhibitor SLPI is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid. The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin. SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids and could be useful as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.
Catalog #S0635
ApplicationsSuitable for use in Neutralization, Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionNeutralization: ~1.5ug/ml (ND50) Neutralizes 50% of the activity at 0.125ug/ml of S0635-01B Secretory Leukocyte Protease Inhibitor Recombinant Human inhibition of 0..5ug/ml of Trypsin cleavage of the fluorogenic peptide substrate Mca­RPKPVE­Nval­WRK(Dnp)­NH2 (10uM). The
Western Blot: 0.1ug/ml (S0635-01B)
Immunohistochemistry: 5-15ug/ml Immersion fixed paraffin­embedded sections of human skin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to a26-132 of human SLPI expressed in E. coli (P03973).
SpecificityRecognizes human SLPI in direct ELISA and Western Blot. ~20% crossreactivity with mouse SLPI (ELISA). Antibody neutralizes SLPI biological activity.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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