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S0668-48 Rabbit Anti-Semaphorin 3B (SEMA3B, Semaphorin V, Seam A(V), Sema V)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q13214
Isotype
IgG
Grade
Affinity Purified
Applications
IC IF IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. SEMA 3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Applications
Suitable for use in Immunohistochemistry, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence (IC): 1:40 Western Blot: 1:5000 Immunohistochemistry (Paraffin): 1:100. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa100-200 at the internal portion of human SEMA3B.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SEMA3B. Species Crossreactivity: This antibody reacts with mouse SEMA 3B protein. The immunogen used for this antibody production has 100% homology with human, 92% with mouse, 90% with Zebrafish, and 90% homology with human, mouse, and rat SEMA 3A protein. There is no significant homology with rat protein.
References
1. Tomizawa, Y., et al. PNAS. 98(24):13954-59 (2001)|2. Castro-Rivera, E., et al. PNAS. 101(31): 11432-37 (2004)
USBio References
No references available
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