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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH, Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH,
Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain
Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Semaphorin 3E (previously SemaH) is a secreted protein belonging to the semaphorin family. Semaphorin 3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Like other Semaphorin 3 proteins, the 775 aa human Semaphorin 3E contains a signal sequence, a Semaphorin domain, a C2-like Ig domain and a C-terminal basic domain. Disulfide-linked dimerization and furin-type proteolytic processing increase Semaphorin 3E bioactivity. Mature human and mouse Semaphorin 3E share 90% aa identity.
Catalog #S0668-50
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml using rhSemaphorin 3E
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H223
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa25-775 (Arg557-Arg560) from human Semaphorin 3E expressed in NS0 cells. (O15041)
SpecificityRecognizes human Semaphorin 3E in direct ELISAs and Western Blots. In Western Blots, this antibody shows <10% crossreactivity with rhSemaphorin 3A, -3B, -6A, -6B, -6C, -6D, -7A, rmSemaphorin 3C, -3E, -3F.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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