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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH, Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH,
Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain
Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Semaphorin 3E (previously SemaH) is a secreted protein belonging to the semaphorin family. Semaphorin 3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Like other Semaphorin 3 proteins, the 775 aa human Semaphorin 3E contains a signal sequence, a Semaphorin domain, a C2-like Ig domain and a C-terminal basic domain. Disulfide-linked dimerization and furin-type proteolytic processing increase Semaphorin 3E bioactivity. Mature human and mouse Semaphorin 3E share 90% aa identity.
Catalog #S0668-50A
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells.
Immunohistochemistry: 10ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos.
Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived rhSemaphorin 3E
SpecificityRecognizes human Semaphorin 3E.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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