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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, KIAA0331, SEMAH)

Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, KIAA0331, SEMAH)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH IC
Semaphorin 3E is one of six Class 3 (secreted) semaphorins which in the human share 40-50% aa identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development. Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Crystal structures of semaphorins reveal that the 500aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure. C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kD form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential. Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins. Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity. Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance.
Catalog #S0668-50H
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa25-775 from human Semaphorin 3E expressed in NSO cell line.
SpecificityRecognizes human Semaphorin 3E. Species sequence homology: Recombinant mouse Semaphorin 3E (70%), mSemaphorin 3C and rmSemaphorin 3F (5%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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