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You are here:Home » Antibodies » Antibodies-Plexin, Semaphorin » Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, KIAA0331, SEMAH)

Anti -Semaphorin 3E (Semaphorin-3E, SEMA3E, KIAA0331, SEMAH)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH IC
Semaphorin 3E is one of six Class 3 (secreted) semaphorins which in the human share 40-50% aa identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development. Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Crystal structures of semaphorins reveal that the 500aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure. C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kD form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential. Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins. Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity. Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance.
Catalog #S0668-50H
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa25-775 from human Semaphorin 3E expressed in NSO cell line.
SpecificityRecognizes human Semaphorin 3E. Species sequence homology: Recombinant mouse Semaphorin 3E (70%), mSemaphorin 3C and rmSemaphorin 3F (5%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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